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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which ends up in chromosome instability, and thus might add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been claimed but comprehensive nucleotide sequence details isn't however offered.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guideline outlines more achievable optimization of cellular and immunoassay parameters to ensure the very best results are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best effects are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the goal protein. By comparing the staining from your blocked antibody as opposed to the antibody by itself, you can see which staining is precise.
Concentrate on Information Cyclin E belongs towards the hugely conserved cyclin family, whose users are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation patterns which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E varieties a complex with and functions to be a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates on the G1-S period boundary and is degraded as cells development via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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